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pbluescript sk(−) phagemid vector  (Agilent technologies)


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    Structured Review

    Agilent technologies pbluescript sk(−) phagemid vector

    Pbluescript Sk(−) Phagemid Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+sk++phagemid+vector/pmc10758284-251-1-5
    Average 90 stars, based on 1 article reviews
    pbluescript sk(−) phagemid vector - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "DNA cleavage assay for the identification of topoisomerase I inhibitors"

    Article Title: DNA cleavage assay for the identification of topoisomerase I inhibitors

    Journal: Nature protocols

    doi: 10.1038/nprot.2008.174


    Figure Legend Snippet:

    Techniques Used:

    Related Articles

    Clone Assay:

    Article Title: Cloning of soybean genes induced during hypersensitive cell death caused by syringolide elicitor.
    Article Snippet: Syringolide elicitors produced by bacteria expressing Pseudomonas syringae pv. glycinea avirulence gene D (avrD) induce hypersensitive cell death (HCD) only in soybean (Glycine max [L.] Merr.) plants carrying the Rpg4 disease resistance gene.. Employing a differential display method, we isolated 13 gene fragments induced in cultured cells of a soybean cultivar Harosoy (Rpg4) treated with syringolides.. Several genes for isolated fragments were induced by syringolides in an rpg4 cultivar Acme as well as in Harosoy; however, the genes for seven fragments designated as SIH (for syringolideinduced/HCD associated) were induced exclusively or strongly in Harosoy. cDNA clones for SIH genes were obtained from a cDNA library of Harosoy treated with syringolide.

    Article Title: SLC18A2 promoter haplotypes and identification of a novel protective factor against alcoholism.
    Article Snippet: .. A 5.4 kb BamHI/Not I DNA fragment was freed from this clone and cloned into a pBluescript SK phagemid vector (Stratagene). ..

    Plasmid Preparation:

    Article Title: Cloning of soybean genes induced during hypersensitive cell death caused by syringolide elicitor.
    Article Snippet: Syringolide elicitors produced by bacteria expressing Pseudomonas syringae pv. glycinea avirulence gene D (avrD) induce hypersensitive cell death (HCD) only in soybean (Glycine max [L.] Merr.) plants carrying the Rpg4 disease resistance gene.. Employing a differential display method, we isolated 13 gene fragments induced in cultured cells of a soybean cultivar Harosoy (Rpg4) treated with syringolides.. Several genes for isolated fragments were induced by syringolides in an rpg4 cultivar Acme as well as in Harosoy; however, the genes for seven fragments designated as SIH (for syringolideinduced/HCD associated) were induced exclusively or strongly in Harosoy. cDNA clones for SIH genes were obtained from a cDNA library of Harosoy treated with syringolide.

    Article Title: SLC18A2 promoter haplotypes and identification of a novel protective factor against alcoholism.
    Article Snippet: .. A 5.4 kb BamHI/Not I DNA fragment was freed from this clone and cloned into a pBluescript SK phagemid vector (Stratagene). ..

    Sequencing:

    Article Title: Small Espin: A Third Actin-bundling Protein and Potential Forked Protein Ortholog in Brush Border Microvilli
    Article Snippet: The amount of actin present in the isolated small intestinal brush borders was determined by scanning laser densitometric analysis of Coomassie blue– stained SDS gels and comparison to a standard curve generated from internal standards containing known amounts of rabbit skeletal muscle actin (Cytoskeleton, Inc., Denver, CO). .. A cDNA (1,088-bp) that included the full coding sequence of the small isoform of espin was obtained using the affinity-purified rabbit polyclonal antibodies to screen a 5′-STRETCH rat kidney λgt11 cDNA library ( CLONTECH Laboratories, Palo Alto, CA). cDNAs were introduced into the pBluescript SK phagemid vector (Stratagene, La Jolla, CA), and the coding and noncoding strands were sequenced in their entirety using [ 35 S]dATPαS and the Sequenase Version 2.0 kit ( Amersham Corp. , Arlington Heights, IL). .. Sequence analysis and database searching were carried out using the programs available through the University of Wisconsin Genetics Computer Group (GCG) Sequence Analysis Software Package ( ) and the BLAST Search ( ) available through the National Center for Biotechnology Information.

    Affinity Purification:

    Article Title: Small Espin: A Third Actin-bundling Protein and Potential Forked Protein Ortholog in Brush Border Microvilli
    Article Snippet: The amount of actin present in the isolated small intestinal brush borders was determined by scanning laser densitometric analysis of Coomassie blue– stained SDS gels and comparison to a standard curve generated from internal standards containing known amounts of rabbit skeletal muscle actin (Cytoskeleton, Inc., Denver, CO). .. A cDNA (1,088-bp) that included the full coding sequence of the small isoform of espin was obtained using the affinity-purified rabbit polyclonal antibodies to screen a 5′-STRETCH rat kidney λgt11 cDNA library ( CLONTECH Laboratories, Palo Alto, CA). cDNAs were introduced into the pBluescript SK phagemid vector (Stratagene, La Jolla, CA), and the coding and noncoding strands were sequenced in their entirety using [ 35 S]dATPαS and the Sequenase Version 2.0 kit ( Amersham Corp. , Arlington Heights, IL). .. Sequence analysis and database searching were carried out using the programs available through the University of Wisconsin Genetics Computer Group (GCG) Sequence Analysis Software Package ( ) and the BLAST Search ( ) available through the National Center for Biotechnology Information.

    cDNA Library Assay:

    Article Title: Small Espin: A Third Actin-bundling Protein and Potential Forked Protein Ortholog in Brush Border Microvilli
    Article Snippet: The amount of actin present in the isolated small intestinal brush borders was determined by scanning laser densitometric analysis of Coomassie blue– stained SDS gels and comparison to a standard curve generated from internal standards containing known amounts of rabbit skeletal muscle actin (Cytoskeleton, Inc., Denver, CO). .. A cDNA (1,088-bp) that included the full coding sequence of the small isoform of espin was obtained using the affinity-purified rabbit polyclonal antibodies to screen a 5′-STRETCH rat kidney λgt11 cDNA library ( CLONTECH Laboratories, Palo Alto, CA). cDNAs were introduced into the pBluescript SK phagemid vector (Stratagene, La Jolla, CA), and the coding and noncoding strands were sequenced in their entirety using [ 35 S]dATPαS and the Sequenase Version 2.0 kit ( Amersham Corp. , Arlington Heights, IL). .. Sequence analysis and database searching were carried out using the programs available through the University of Wisconsin Genetics Computer Group (GCG) Sequence Analysis Software Package ( ) and the BLAST Search ( ) available through the National Center for Biotechnology Information.



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    Image Search Results


    Journal: Nature protocols

    Article Title: DNA cleavage assay for the identification of topoisomerase I inhibitors

    doi: 10.1038/nprot.2008.174

    Figure Lengend Snippet:

    Article Snippet: REAGENTS pBluescript SK(−) Phagemid Vector (Stratagene, cat. no. 212206).

    Techniques:

    cDNA Library Attributes

    Journal: BMC Plant Biology

    Article Title: Identification of tissue-specific, abiotic stress-responsive gene expression patterns in wine grape ( Vitis vinifera L.) based on curation and mining of large-scale EST data sets

    doi: 10.1186/1471-2229-11-86

    Figure Lengend Snippet: cDNA Library Attributes

    Article Snippet: The cDNAs were then directionally cloned into EcoRI-NotI digested pBluescript II SK+ phagemid vector (Stratagene, Inc., La Jolla, CA).

    Techniques: cDNA Library Assay, Clone Assay, Sequencing, Plasmid Preparation